CD63 Antikörper

Produktnummer ABIN120614

Der Maus Monoklonal Anti-CD63 Antikörper wurde für FACS, WB, IF, IP und IHC (p) validiert. Er ist geeignet, CD63 in Proben von Human zu detektieren. Es ist 1 Publikation verfügbar.

Kurzübersicht für CD63 Antikörper (ABIN120614)

Target: CD63

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD63 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: MEM-259

Produktdetails anti-CD63 Antikörper

Spezifität: The antibody reacts with CD63 (LAMP-3), a 40-60 kDa tetraspan glycoprotein expressed by granulocytes, platelets, T cells, monocytes/macrophages and endothelial cells. Cell surface exposition of CD63 is usually activation-dependent.

Kreuzreaktivität (Details): Species reactivity (tested):Human.

Aufreinigung: Affinity Chromatography on Protein A

Immunogen: HPB-ALL T cell line

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow cytometry (detection of activated platelets, neutrophils and basophils). Immunoprecipitation. Western blotting under non-reducing conditions. Immunocytochemistry. Immunohistochemistry (paraffin sections): Recommended dilution:10 m g/mL, Positive tissue: spleen
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.4, 15 mM Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch. General.

Haltbarkeit: 12 months

Referenzen für anti-CD63 Antikörper (ABIN120614)

Schmidt, Gelhaus, Nebendahl, Lettau, Lucius, Leippe, Kabelitz, Janssen: "Effector granules in human T lymphocytes: the luminal proteome of secretory lysosomes from human T cells." in: Cell communication and signaling : CCS, Vol. 9, Issue 1, pp. 4, (2011) (PubMed).

Details zu CD63

Target: CD63

Andere Bezeichnung: CD63

Hintergrund: CD63, a glycoprotein of tetraspanin family, is present in late endosomes, lysosomes and secretory vesicles of various cell types. It is also present in the plasma membrane, usually following cell activation. Hence, it has become an widely used basophil activation marker. In mast cells, however, CD63 exposition does not need their activation. CD63 interacts with integrins and affects phagocytosis and cell migration, it is also involved in H/K-ATPase trafficking regulation of ROMK1 channels. CD63 also serves as a T-cell costimulation molecule. Expression of CD63 can be used for predicting the prognosis in earlier stages of carcinomas.Synonyms: Granulophysin, ME491, MLA1, Melanoma-associated antigen ME491, OMA81H, Ocular melanoma-associated antigen, TSPAN30, Tetraspanin-30

Gen-ID: 967

UniProt: P08962